![]() Shackleton, in Patient Derived Tumor Xenograft Models, 2017 Future/Challenges Transgenic mice have been used in neuroAIDS studies to characterize the mechanism of HIV-induced neurotoxicity that may be implemented in HAND. Both HIV-infected and immune-activated macrophages in the CNS release neurotoxins that can induce apoptosis and damage dendrite and synapse function ( Rahimian and He, 2016). NeuroAIDS studies using humanized mice highlighted the importance of Mo/MΦ infection and their association with neuronal injury in the development of HIV-associated encephalitis ( Gorantla et al., 2010 Dash et al., 2011 Honeycutt et al., 2016). This important study underscores the pivotal role of macrophages in AIDS and HIV-associated CNS pathology ( Honeycutt et al., 2016). A recent study using this model, termed the “macrophage-only mice,” demonstrated that Mo/MΦs alone can sustain HIV infection in the absence of CD4 + T cells. NSG mice that are transplanted with human hematopoietic stem cells or BM without human thymus and liver tissue will develop mature human myeloid, but not lymphoid, cells ( Honeycutt et al., 2016). NSG mice are a useful model for studying specific immune responses and infected cells implicated in HIV-associated neuropathogenesis ( Sun et al., 2007 Lavender et al., 2013). Williams, in Handbook of Clinical Neurology, 2018 T-cell and macrophage-only humanized mice Animals bearing the Raji cells were subsequently randomized into different groups with half males and half females according to their body weights or fluorescence intensity. If a Raji cell with fluorescence reporter was used, the fluorescence intensity of animals for the proliferation of the lymphoma could be detected 72–96 h after xenografting using IVIS Lumina III (Perkin Elmer). Before the study was conducted, mice were quarantined for 5 days.Īnimals to be administrated with CAR-T were intravenously dosed with 0.2 mL Raji cells (3 ~ 5 × 10 5 cells per animal) for tumor xenografts in advance. Animals had ad libitum access to certified rodent diet, and sterilized municipal tap water was given ad libitum via water bottles. #Nod scid gamma mice freeSix-week-old specific pathogen free (SPF) NSG mice were housed in individually ventilated cages in a barrier system under conditions of 20–26 ☌, 40–70% relative humidity, a 12-h light–dark cycle, and a cage air exchange of over 50 times per hour. NSG mice with IL2rg gene knockout and NOD- scid genetic background, lacking mature T, B and NK cells, are recognized as a suitable immunodeficient model for the cell xenografting. Yan Huo, in Methods in Cell Biology, 2022 2.2 Animals and tumor xenografting In conclusion, we demonstrated for the first time that allogeneic HSCs from a different inbred strain can compete for niches in the BM compartment of NSG mice.Hairuo Wen. Using this novel NSG transplantation model, we will be able to study the effects of low dose in vivo X-ray exposure on the long-term fate of HSCs, without the requirement of prior radio-ablation of the recipient, and thus leaving the recipient’s BM microenvironment uncompromised. Also, in vivo irradiated HSCs showed long-term engraftment, although overall white blood cell (WBC) donor chimerism was lower compared with non-irradiated HSCs. All transplanted NSG mice showed long-term myeloid and lymphoid cell chimerism. We transplanted allogeneic HSCs constitutively expressing the mCherry fluorescent marker into age-matched NSG mice and assessed donor chimerism 6 months post-transplantation. Here, we present data on the transplantation of HSCs into NOD scid gamma (NSG) mice to achieve long-term engraftment without prior conditioning. Nevertheless, NOD scid mice have a significantly altered life span due to early development of thymic lymphomas, which compromises the ability to study the long-term fate of exogenous HSCs and their progeny. ![]() Scid hematopoietic stem cells (HSCs) have an intrinsic defect in their maintenance within the bone marrow (BM) niche which facilitates HSC transplantation without the absolute requirement of prior conditioning. ![]()
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